DLC films as material suitable for adhesion of biomolecules from Luria Bertani medium

0486995 - FZÚ 2018 RIV CZ eng A - Abstract
Artemenko, Anna - Romanyuk, Olexandr - Matyska Lišková, P. - Gordeev, Ivan - Jiříček, Petr - Beranová, J. - Hruška, Karel - Marton, M. - Ukraintsev, Egor - Kromka, Alexander
DLC films as material suitable for adhesion of biomolecules from Luria Bertani medium.
Nanoworkshop 2017- Book of abstracts of the International Workshop on Polymer Metal Nanocomposites /8./. Praha, 2017. OR3. ISBN 978-80-7378-347-1.
[Nanoworkshop 2017 - International Workshop on Polymer Metal Nanocomposites /8./. 12.09.2017-15.09.2017, Praha]
R&D Projects: GA MŠMT LM2015088; GA ČR GA15-01687S
Institutional support: RVO:68378271
Keywords : diamond * AFM * XPS
OECD category: Biophysics

Diamond-like carbon (DLC) films are promising for medical applications, especially due to their antibacterial properties. In this work, the adhesion of biomolecules from complex cultivation medium Luria Bertani (LB) on surface terminated DLC films is investigated using X-ray photoelectron spectroscopy (XPS).The DLC films were deposited by DC PACVD method. Their surface termination by H, O or F was carried out by RF plasma in appropriate gas atmosphere (hydrogen, oxygen, carbon tetrafluoride). All functionalized samples were sterilized in autoclave before immersion into LB medium. AFM measurements confirmed the adhesion of ~2 nm thin peptide layer on LB-treated samples. XPS measurements revealed the differences in NH2/NH3 ratios for LB-treated glass and LB-DLC films. In case of oxygen, the dependence of O-H/C=O bond ratio on the surface termination was observed. These indicate that the adhesion of biomolecules from LB medium can be affected by DLC surface termination.
Permanent Link: http://hdl.handle.net/11104/0281694