Identification of genetic elements in metabolism by high-throughput mouse phenotyping

0486935 - ÚMG 2018 RIV GB eng J - Journal Article
Sedláček, Radislav … Total 61 authors
Identification of genetic elements in metabolism by high-throughput mouse phenotyping.
Nature Communications. Roč. 9, zima (2018), č. článku 288. E-ISSN 2041-1723
Institutional support: RVO:68378050
Keywords : Insulin-resistance * Diabetes-mellitus * Glycemic traits * Variants * Architecture * Association * Consortiuj * Pathways * Disease * Biology
OECD category: Genetics and heredity (medical genetics to be 3)
Impact factor: 11.878, year: 2018

Metabolic diseases are a worldwide problem but the underlying genetic factors and their relevance to metabolic disease remain incompletely understood. Genome-wide research is needed to characterize so-far unannotated mammalian metabolic genes. Here, we generate and analyze metabolic phenotypic data of 2016 knockout mouse strains under the aegis of the International Mouse Phenotyping Consortium (IMPC) and find 974 gene knockouts with strong metabolic phenotypes. 429 of those had no previous link to metabolism and 51 genes remain functionally completely unannotated. We compared human orthologues of these uncharacterized genes in five GWAS consortia and indeed 23 candidate genes are associated with metabolic disease. We further identify common regulatory elements in promoters of candidate genes. As each regulatory element is composed of several transcription factor binding sites, our data reveal an extensive metabolic phenotype-associated network of coregulated genes. Our systematic mouse phenotype analysis thus paves the way for full functional annotation of the genome.
Permanent Link: http://hdl.handle.net/11104/0281640