Impact of intestinal microbiota on mice behavior in chronic psychosocial stress model

0484613 - MBÚ 2018 NL eng A - Abstract
Hermanová, Petra - Vodička, Martin - Ergang, Peter - Mikulecká, Anna - Kozáková, Hana - Pácha, Jiří - Hudcovic, Tomáš
Impact of intestinal microbiota on mice behavior in chronic psychosocial stress model.
Mind, mood & microbes, Abstracts of lectures and posters. Amstedam: The 1st international konference on microbiota-gut-brain axis, 2016. s. 73.
[The 1st international konference on microbiota-gut-brain axis. 01.12.2016-02.12.2016, Amsterdam]
R&D Projects: GA ČR GA15-07268S
Institutional support: RVO:61388971
Keywords : Stress * HPA axis * GF * SPF
OECD category: Immunology

The intestinal microbiota is complex ecosystem closely linked with health and disease of the host. Data have shown that microbiota is able to modulate normal function of the nervous system but the precise mechanism is still under investigation. Mounting evidence also support the theory that it is just intestinal microbiota which plays a crucial role in early programming and also later responsivity of the stress system. Aim of our study was to establish a mouse model of chronic psychosocial stress in germ-free (GF) and specific pathogen-free (SPF) conditions using males of Balb/c mice. Experiments were carried out in the Trexler-like isolators. GF and SPF mice were treated under the same isolator conditions. The model of resident-intruder was used in our experiment to induce stress response. Young male, intruder, was placed into the cage of unfamiliar older male, resident. 10 minutes of free interaction was followed by 50 minutes during which the mice were separated by a steel grate. First 10 minutes of free interaction was video-recorded and patterns of defensive behavior were analyzed – fly, upright posture, freezing, escape/ flight. Time spent in mentioned patterns was assessed. The differences in behavior of GF and SPF mice were observed. We concluded that intestinal microbiota has impact on behavior of mice in chronic psychosocial stress model.
Permanent Link: http://hdl.handle.net/11104/0279775