The role of probiotic strain Escherichia coli Nissle 1917 in gnotobiotic mice experimental models.

0484605 - MBÚ 2018 NL eng A - Abstract
Hudcovic, Tomáš - Štěpánková, Renata - Kozáková, Hana - Šrůtková, Dagmar - Schwarzer, Martin - Hermanová, Petra - Šestáková, Blanka - Tlaskalová-Hogenová, Helena
The role of probiotic strain Escherichia coli Nissle 1917 in gnotobiotic mice experimental models.
Abstracts of lectures and posters The 6th Beneficial Microbes Conference. Amsterdam: Conference secretariat, 2017.
[6th Beneficial Microbes Conference, Pre-and Probiotics for lifelong human and animal health. 09.10.2017-11.10.2017, Amsterdam]
R&D Projects: GA ČR GA15-07268S
Institutional support: RVO:61388971
Keywords : gnotobiotic mice * E. coli Nissle 1917 * E. coli O6K13 * cytokine
OECD category: Immunology

To study the effect of E. coli strains on the development of acute ulcerative colitis (UC) induced by dextran sulfate sodium (DSS) in gnotobiotic mice.
Mice monoassociated with E. coli Nissle 1917 strain and then reassociated by E. coli O6K13 strain developed lower degree of intestinal inflammation in colon in DSS model of ulcerative colitis than conversely associated group. In the first group, the level of pro-inflammatory cytokine TNF-alpha and IL-6, ZO-1 and IgA was reduced markedly in colon compared to the second experimental group.We conclude that E. coli Nissle 1917 colonization protects mice against intestinal inflammation induced by E. coli O6K13 strain in DSS model of ulcerative colitis. For this reason, we believe that E. coli Nissle 1917 is a suitable candidate, which we will further test in other gnotobiotic models.

Permanent Link: http://hdl.handle.net/11104/0279771