Chondrogenic potential of macroporous biodegradable cryogels based on synthetic poly(.alpha.-amino acids)

0483972 - ÚMCH 2019 RIV GB eng J - Journal Article
Sedlačík, Tomáš - Acar, O. K. - Studenovská, Hana - Kotelnikov, Ilya - Kučka, Jan - Konečná, Z. - Zikmund, T. - Kaiser, J. - Köse, G. T. - Rypáček, František
Chondrogenic potential of macroporous biodegradable cryogels based on synthetic poly(.alpha.-amino acids).
Soft Matter. Roč. 14, č. 2 (2018), s. 228-238. ISSN 1744-683X. E-ISSN 1744-6848
Institutional support: RVO:61389013
Keywords : poly(amino acids) * cryogelation * scaffold
OECD category: Polymer science
Impact factor: 3.399, year: 2018

In this study, the potential of highly porous hydrogels based on biodegradable synthetic poly(α-amino acids) to support proliferation and chondrogenesis of human dental pulp stem cells (hDPSCs) was investigated. Covalently crosslinked gels with permanent pores were formed under cryogenic conditions by free-radical copolymerization of poly[N5-(2-hydroxyethyl)-L-glutamine-stat-N5-(2-methacryloyl-oxy-ethyl)-L-glutamine] (PHEG-MA) with 2-hydroxyethyl methacrylate (HEMA) and N-propargyl methacrylamide (PrMAAm) as minor co-monomers. PrMAAm provided alkyne groups for modifying the gels with cell-supporting moieties (RGDS peptides) by the azide–alkyne “click”-reaction. Two types of gels with different compressive moduli were prepared. Each type was modified with two different concentrations of RGDS peptide. X-ray computed nanotomography (nanoCT) was used to visualize and analyze the 3D-structure of the cryogels. It was shown that modifying the PHEG-MA cryogels within the range of RGDS concentrations examined here had a positive effect on the proliferation of hDPSCs. Immunofluorescence staining for collagen type 2 and aggrecan proved that there was differentiation of hDPSCs into chondrocytes.
Permanent Link: http://hdl.handle.net/11104/0282594