Non-invasive and invasive diagnoses of aspergillosis in a rat model by mass spectrometry

0483806 - MBÚ 2018 RIV GB eng J - Journal Article
Luptáková, Dominika - Pluháček, Tomáš - Petřík, M. - Novák, Jiří - Palyzová, Andrea - Sokolová, Lucie - Škríba, Anton - Šedivá, Blanka - Lemr, Karel - Havlíček, Vladimír
Non-invasive and invasive diagnoses of aspergillosis in a rat model by mass spectrometry.
Scientific Reports. Roč. 7, NOV 28 (2017), č. článku 16523. ISSN 2045-2322. E-ISSN 2045-2322
R&D Projects: GA MŠMT(CZ) LO1509; GA ČR(CZ) GA16-20229S
Institutional support: RVO:61388971
Keywords : FUMIGATUS * SIDEROPHORES * GA-68-SIDEROPHORES
OECD category: Analytical chemistry
Impact factor: 4.122, year: 2017

Invasive pulmonary aspergillosis results in 450,000 deaths per year and complicates cancer chemotherapy, transplantations and the treatment of other immunosuppressed patients. Using a rat model of experimental aspergillosis, the fungal siderophores ferricrocin and triacetylfusarinine C were identified as markers of aspergillosis and quantified in urine, serum and lung tissues. Biomarkers were analyzed by matrix-assisted laser desorption ionization (MALDI) and electrospray ionization mass spectrometry using a 12T SolariX Fourier transform ion cyclotron resonance (FTICR) mass spectrometer. The limits of detection of the ferri-forms of triacetylfusarinine C and ferricrocin in the rat serum were 0.28 and 0.36 ng/mL, respectively. In the rat urine the respective limits of detection achieved 0.02 and 0.03 ng/mL. In the sera of infected animals, triacetylfusarinine C was not detected but ferricrocin concentration fluctuated in the 3-32 ng/mL range. Notably, the mean concentrations of triacetylfusarinine C and ferricrocin in the rat urine were 0.37 and 0.63 mu g/mL, respectively. The MALDI FTICR mass spectrometry imaging illustrated the actual microbial ferricrocin distribution in the lung tissues and resolved the false-positive results obtained by the light microscopy and histological staining. Ferricrocin and triacetylfusarinine C detection in urine represents an innovative non-invasive indication of Aspergillus infection in a host.
Permanent Link: http://hdl.handle.net/11104/0279001