Long non-coding RNA exchange during the oocyte-to-embryo transition in mice

0483739 - ÚMG 2018 RIV GB eng J - Journal Article
Karlic, R. - Ganesh, Sravya - Franke, V. - Svobodová, Eliška - Urbanová, Jana - Suzuki, Y. - Aoki, F. - Vlahovicek, K. - Svoboda, Petr
Long non-coding RNA exchange during the oocyte-to-embryo transition in mice.
Dna Research. Roč. 24, č. 2 (2017), s. 129-141. ISSN 1340-2838. E-ISSN 1756-1663
R&D Projects: GA ČR(CZ) GBP305/12/G034; GA MŠMT LO1419; GA MŠMT(CZ) LM2011032; GA MŠMT(CZ) LM2015040; GA MŠMT(CZ) ED1.1.00/02.0109
Institutional support: RVO:68378050
Keywords : IncRNA * oocyte * zygote * polyadenylation * endo-siRNA
OECD category: Reproductive biology (medical aspects to be 3)
Impact factor: 5.415, year: 2017

The oocyte-to-embryo transition (OET) transforms a differentiated gamete into pluripotent blastomeres. The accompanying maternal-zygotic RNA exchange involves remodeling of the long non-coding RNA (lncRNA) pool. Here, we used next generation sequencing and de novo transcript assembly to define the core population of 1,600 lncRNAs expressed during the OET (lncRNAs). Relative to mRNAs, OET lncRNAs were less expressed and had shorter transcripts, mainly due to fewer exons and shorter 5' terminal exons. Approximately half of OET lncRNA promoters originated in retrotransposons suggesting their recent emergence. Except for a small group of ubiquitous lncRNAs, maternal and zygotic lncRNAs formed two distinct populations. The bulk of maternal lncRNAs was degraded before the zygotic genome activation. Interestingly, maternal lncRNAs seemed to undergo cytoplasmic polyadenylation observed for dormant mRNAs. We also identified lncRNAs giving rise to trans-acting short interfering RNAs, which represent a novel lncRNA category. Altogether, we defined the core OET lncRNA transcriptome and characterized its remodeling during early development. Our results are consistent with the notion that rapidly evolving lncRNAs constitute signatures of cells-of-origin while a minority plays an active role in control of gene expression across OET. Our data presented here provide an excellent source for further OET lncRNA studies.
Permanent Link: http://hdl.handle.net/11104/0278943