Chronic disruptions of circadian sleep regulation induce specific proinflammatory responses in the rat colon

0483638 - FGÚ 2018 RIV US eng J - Journal Article
Polidarová, Lenka - Houdek, Pavel - Sumová, Alena
Chronic disruptions of circadian sleep regulation induce specific proinflammatory responses in the rat colon.
Chronobiology International. Roč. 34, č. 9 (2017), s. 1273-1287. ISSN 0742-0528. E-ISSN 1525-6073
R&D Projects: GA ČR(CZ) GA14-07711S
Institutional support: RVO:67985823
Keywords : aging * colon * constant light * melatonin * proinflammatory cytokine * Rgs16 * sleep disruption
OECD category: Physiology (including cytology)
Impact factor: 2.643, year: 2017

Exposure to environmental conditions that disturb the daily rhythms has been shown to enhance the proinflammatory responses of immunostimulant-challenged immune system. However, it is not known whether circadian disturbances may stimulate unchallenged immune responses and thus contribute per se to the development of inflammation-related diseases. Our aim was to ascertain an effect of various conditions threatening the behavioral activity/rest cycle regulation, namely aging with or without melatonin, 6-h advance/delay phase shifts in the light/dark cycle repeated with a 2-day frequency and constant light, on expression of immune markers in the rat colon. We detected impact of these conditions on parameters of behavioral activity and mRNA levels of selected immune markers in the colonic mucosa of Wistar rats, namely TNFa (Tnf), IL1a (Il1a), IL17RA (Il17ra), STAT3 (Stat3), and Rgs16 (Rsg16), were detected. Exposure to constant light, which perturbed the interval of inactivity (sleep) and led to the complete abolishment of activity/inactivity cycles, activated robustly proinflammatory state in the colon selectively via Stat3-dependent pathway. These results provided the first evidence that disruptions in the behavioral activity/inactivity cycles may spontaneously (without immuno-stimulant) induce selective proinflammatory responses in the colonic mucosa. Such effects may take part in the mechanisms of modern lifestyle-induced inflammatory diseases of the gut.
Permanent Link: http://hdl.handle.net/11104/0278852