0483621 - FZÚ 2018 RIV NL eng J - Journal Article
Angelov, Borislav - Garamus, V.M. - Drechsler, M. - Angelova, A.
Structural analysis of nanoparticulate carriers for encapsulation of macromolecular drugs.
Journal of Molecular Liquids. Roč. 235, Jun (2017), s. 83-89. ISSN 0167-7322. E-ISSN 1873-3166
R&D Projects: GA MŠMT EF15_003/0000447; GA MŠMT EF15_008/0000162
Grant - others:OP VVV - ELIBIO(XE) CZ.02.1.01/0.0/0.0/15_003/0000447; ELI Beamlines(XE) CZ.02.1.01/0.0/0.0/15_008/0000162
Institutional support: RVO:68378271
Keywords : self-assembled nanocarriers * liquid crystalline phase transitions * cationic lipids * macromolecular drugs
OECD category: Biophysics
Impact factor: 4.513, year: 2017

Recently developed macromolecular drugs display strong potential to cure diseases involving genetic components, e.g. Rheumatoid arthritis, diabetes, Alzheimer's disease, Huntington's disease, Duchenne muscular dystrophy, Retinitis pigmentosa, and several types of cancers. Bioavailability and enhanced drug specificity necessitate the preparation of efficient carrier systems for the macromolecular therapeutics. We employ synchrotron small angle X-ray scattering (SAXS) and cryogenic transmission electron microscopy (Cryo-TEM) to study the nano structure formation, macromolecular drug upload, and the topological transformations occurring upon complexation of supercoiled plasmid DNA (encoding for the therapeutic protein brain-derived neurotrophic factor, BDNF) with cationic lipid nanocarrier assemblies.
Permanent Link: http://hdl.handle.net/11104/0278844