Number of the records: 1
3 days Hands-on qPCR
- 1.0483576 - BTÚ 2018 RIV eng U - Conference, Workshop Arrangement
Kubista, Mikael - Šindelka, Radek - Valihrach, Lukáš - Tománková, Silvie - Androvič, Peter - Rohlová, Eva - Švec, David - Langerová, Lucie
3 days Hands-on qPCR.
[Vestec, BTÚ, 27.03.2017-29.03.2017, (W-WRD 10/4)]
R&D Projects: GA MŠMT(CZ) ED1.1.00/02.0109
Institutional support: RVO:86652036
Keywords : qPCR * biostatistic * reverese transcription
OECD category: Genetics and heredity (medical genetics to be 3)
Day 1. Introduction to PCR and qPCR. qPCR data evaluation. Primer and probe design. Optimization of qPCR protocols. Hands-on lab running qPCR analyzing unknown samples with standard curve. Day 2. Normalization. Basic quantification theory. Absolute quantification strategies. Validation of assays. Quantification calculation examples. Hands-on lab running qPCR and calculating relative quantities with different strategies. Day 3. Sample preparation and isolation. Reverse transcription, RT. The MIQE guidelines. Group discussion
Permanent Link: http://hdl.handle.net/11104/0278829
Number of the records: 1