Yeast phospholipase C is required for stability of casein kinase I Yck2p and expression of hexose transporters

0483264 - MBÚ 2018 RIV GB eng J - Journal Article
Zhang, T. - Galdieri, L. - Hašek, Jiří - Vančura, A.
Yeast phospholipase C is required for stability of casein kinase I Yck2p and expression of hexose transporters.
FEMS Microbiology Letters. Roč. 364, č. 22 (2017), č. článku fnx227. ISSN 0378-1097. E-ISSN 1574-6968
R&D Projects: GA ČR(CZ) GA16-05497S
Institutional support: RVO:61388971
Keywords : phospholipase C * casein kinase I * hexose transporters
OECD category: Microbiology
Impact factor: 1.735, year: 2017

Phospholipase C (Plc1p) in Saccharomyces cerevisiae is required for normal degradation of repressor Mth1p and expression of the HXT genes encoding cell membrane transporters of glucose. Plc1p is also required for normal localization of glucose transporters to the cell membrane. Consequently, plc1 Delta cells display histone hypoacetylation and transcriptional defects due to reduced uptake and metabolism of glucose to acetyl-CoA, a substrate for histone acetyltransferases. In the presence of glucose, Mth1p is phosphorylated by casein kinase I Yck1/2p, ubiquitinated by the SCFGrr1 complex and degraded by the proteasome. Here, we show that while Plc1p does not affect the function of the SCFGrr1 complex or the proteasome, it is required for normal protein level of Yck2p. Since stability of Yck1/2p is regulated by a glucose-dependent mechanism, PLC1 inactivation results in destabilization of Yck1/2p and defect in Mth1p degradation. Based on our results and published data, we propose a model in which plc1 Delta mutation causes increased internalization of glucose transporters, decreased transport of glucose into the cells, and consequently decreased stability of Yck1/2p, increased stability of Mth1p and decreased expression of the HXT genes.
Permanent Link: http://hdl.handle.net/11104/0278630