A microfluidic device for investigation of migratory potential of cancer cells

0483170 - ÚIACH 2018 GB eng A - Abstract
Klepárník, Karel - Ledvina, Vojtěch - Balvan, J. - Raudenská, M. - Gumulec, J. - Masařík, M.
A microfluidic device for investigation of migratory potential of cancer cells.
4th 4BIO Summit. 2017. s. 1-2.
[Microfluidics congress Europe /3./. 04.12.2017-05.12.2017, London]
R&D Projects: GA ČR(CZ) GA17-01995S
Institutional support: RVO:68081715
Subject RIV: CB - Analytical Chemistry, Separation

Functionality of a microfluidic device made of polydimethylsiloxane for testing epithelial-mesenchymal transition of cancer cells is presented. Arrays of micropillars as obstacles with different spacing are a prerequisite of high throughput measurements and standard evaluations of cellular deformability and migratory activity. The miniaturization of the device with the total migration path length less than 2 mm shortened analysis time by two orders of magnitude, when compared with commonly used devices. The device is compatible with multimodal holographic microscope in a time-lapse setup to visualize variability of mechanical properties of many individual cells under identical conditions. This arrangement enables observations unaffected by artifacts caused by phase contrast, due to a fluorescent staining or intensive light fluxes. Cells were subsequently monitored for several hours using holographic microscope Q-Phase (Tescan) with objectives Nikon Plan 10/0.30. Holograms were captured by CCD camera (MC-VELETA).
Permanent Link: http://hdl.handle.net/11104/0278565