Nanoparticle core stability and surface functionalization drive the mTOR signaling pathway in hepatocellular cell lines

0482386 - FZÚ 2018 RIV GB eng J - Journal Article
Lunova, Mariia - Prokhorov, Andriy - Jirsa, M. - Hof, Martin - Olžyńska, Agnieszka - Jurkiewicz, Piotr - Kubinová, Šárka - Lunov, Oleg - Dejneka, Alexandr
Nanoparticle core stability and surface functionalization drive the mTOR signaling pathway in hepatocellular cell lines.
Scientific Reports. Roč. 7, Nov (2017), s. 1-16, č. článku 16049. ISSN 2045-2322. E-ISSN 2045-2322
R&D Projects: GA MŠMT LO1409; GA MŠMT LM2015088
Grant - others:AV ČR(CZ) Fellowship J. E. Purkyně
Institutional support: RVO:68378271 ; RVO:61388955
Keywords : nanoparticle core stability * surface functionalization drive * mTOR signaling pathway * hepatocellular cell lines
OECD category: Biophysics; Physical chemistry (UFCH-W)
Impact factor: 4.122, year: 2017

Specifically designed and functionalized nanoparticles hold great promise for biomedical applications. Yet, the applicability of nanoparticles is critically predetermined by their surface functionalization and biodegradability. Here we demonstrate that amino-functionalized polystyrene nanoparticles (PS-NH2), but not amino- or hydroxyl-functionalized silica particles, trigger cell death in hepatocellular carcinoma Huh7 cells. Importantly, biodegradability of nanoparticles plays a crucial role in regulation of essential cellular processes. Thus, biodegradable silica nanoparticles having the same shape, size and surface functionalization showed opposite cellular effects in comparison with similar polystyrene nanoparticles. At the molecular level, PS-NH2 obstruct and amino-functionalized silica nanoparticles (Si-NH2) activate the mTOR signalling in Huh7 and HepG2 cells.
Permanent Link: http://hdl.handle.net/11104/0277783