Morphological and Production Changes in Planktonic and Biofilm Cells Monitored Using SEM and Raman Spectroscopy

0482373 - ÚPT 2018 RIV US eng J - Journal Article
Hrubanová, Kamila - Krzyžánek, Vladislav - Samek, Ota - Skoupý, Radim - Šiler, Martin - Ježek, Jan - Obruča, S. - Zemánek, Pavel
Morphological and Production Changes in Planktonic and Biofilm Cells Monitored Using SEM and Raman Spectroscopy.
Microscopy and Microanalysis. Roč. 23, S1 (2017), s. 1158-1159. ISSN 1431-9276. E-ISSN 1435-8115
R&D Projects: GA ČR(CZ) GA15-20645S; GA ČR(CZ) GA16-12477S; GA ČR(CZ) GA17-15451S; GA MŠMT(CZ) LO1212; GA MŠMT ED0017/01/01
Institutional support: RVO:68081731
Keywords : biofilm cells * SEM * Raman spectroscopy
OECD category: Electrical and electronic engineering
Impact factor: 2.124, year: 2017

Many microorganisms (e.g., bacteria, yeast, algae) are known to form a multilayered structure composed of cells and extracellular matrix. Such a formation is known as the biofilm. In this contribution we focus
on the bacteria Burkholderia sacchari which accumulates biodegradable polyesters, namely the most common member of polyhydroxyalkanoates (PHAs), poly(3-hydroxybutyrate) (PHB). Therefore, we
investigated planktonic cells and biofilm cells. Elucidation of the basic mechanisms of the biofilm formation and its influence on polyesters formation within the cells would contribute to deeper understanding of the basic biochemical mechanisms underlying this process and developing new and
more efficient strategies for the utilization of bacteria for biotechnological applications.
Permanent Link: http://hdl.handle.net/11104/0277774