Rapid identification of staphylococci by Raman spectroscopy

0480832 - ÚPT 2018 RIV GB eng J - Journal Article
Rebrošová, K. - Šiler, Martin - Samek, Ota - Růžička, F. - Bernatová, Silvie - Holá, V. - Ježek, Jan - Zemánek, Pavel - Sokolová, J. - Petráš, P.
Rapid identification of staphylococci by Raman spectroscopy.
Scientific Reports. Roč. 7, NOV (2017), s. 1-8, č. článku 14846. ISSN 2045-2322. E-ISSN 2045-2322
R&D Projects: GA ČR(CZ) GA15-20645S; GA MŠMT(CZ) LO1212; GA MŠMT ED0017/01/01
Institutional support: RVO:68081731
Keywords : coagulase-negative staphylococci * Raman spectroscopy * rapid identification
OECD category: Optics (including laser optics and quantum optics)
Impact factor: 4.122, year: 2017

Clinical treatment of the infections caused by various staphylococcal species differ depending on the actual cause of infection. Therefore, it is necessary to develop a fast and reliable method for identification of staphylococci. Raman spectroscopy is an optical method used in multiple scientific fields. Recent studies showed that the method has a potential for use in microbiological research, too. Our work here shows a possibility to identify staphylococci by Raman spectroscopy. We present a method that enables almost 100 percent successful identification of 16 of the clinically most important staphylococcal species directly from bacterial colonies grown on a Mueller-Hinton agar plate. We obtained characteristic Raman spectra of 277 staphylococcal strains belonging to 16 species from a 24-hour culture of each strain grown on the Mueller-Hinton agar plate using the Raman instrument. The results show that it is possible to distinguish among the tested species using Raman spectroscopy and therefore it has a great potential for use in routine clinical diagnostics.
Permanent Link: http://hdl.handle.net/11104/0276509