The Limnohabitans genus harbors generalistic and opportunistic subtypes: Evidence from spatiotemporal succession in a canyon-shaped reservoir

0480568 - BC 2018 RIV US eng J - Journal Article
Jezberová, Jitka - Jezbera, Jan - Znachor, Petr - Nedoma, Jiří - Kasalický, Vojtěch - Šimek, Karel
The Limnohabitans genus harbors generalistic and opportunistic subtypes: Evidence from spatiotemporal succession in a canyon-shaped reservoir.
Applied and Environmental Microbiology. Roč. 83, č. 21 (2017), č. článku e01530-17. ISSN 0099-2240. E-ISSN 1098-5336
R&D Projects: GA ČR GA15-12197S; GA ČR(CZ) GA13-00243S
Institutional support: RVO:60077344
Keywords : Limnohabitans * canyon-shaped freshwater reservoir * bacterial diversity * reverse line blot hybridization
OECD category: Microbiology
Impact factor: 3.633, year: 2017

We studied the diversity of Limnohabitans using reverse line blot hybridization with Limnohabitans lineage-specific probes in the freshwater canyonshaped Rimov reservoir (Czech Republic). To examine the succession of distinct lineages, we performed (i) a study of an intensive spring sampling program at the lacustrine part of the Rimov reservoir (from ice melt through a phytoplankton peak to the clear-water phase), and (ii) a seasonal study (April to November) when the occurrence of distinct Limnohabitans lineages was related to the inherent longitudinal heterogeneity of the reservoir. Significant spatiotemporal changes in the compositions of distinct Limnohabitans lineages allowed for the identification of generalists that were always present throughout the whole season as well as specialists that appeared in the reservoir only for limited periods of time or irregularly. Our results indicate that some phytoplankton groups, such as cryptophytes or cyanobacteria, and zooplankton composition were the major factors modulating the distribution and dynamics of distinct Limnohabitans lineages. The highest Limnohabitans diversity was observed during the spring algal bloom, whereas the lowest was during the summer cyanobacterial bloom. The microdiversity also markedly increased upstream in the reservoir, being highest at the inflow, and thus likely reflecting strong influences of the watershed.
Permanent Link: http://hdl.handle.net/11104/0276320