Auxins and Cytokinins in Plant Biology: Methods and Protocols

0480370 - ÚEB 2018 RIV NL eng M - Monography Chapter
Dobrev, Petre - Hoyerová, Klára - Petrášek, Jan
Analytical determination of auxins and cytokinins.
Auxins and Cytokinins in Plant Biology: Methods and Protocols. Dordrecht: Springer, 2017 - (Dandekar, T.; Naseem, M.), s. 31-39. ISBN 978-1-4939-6831-2
R&D Projects: GA MŠMT LD14120; GA MŠMT LD15137
Institutional support: RVO:61389030
Keywords : Auxin * Cytokinins * Liquid chromatography * Mass spectrometry * Phytohormones
OECD category: Analytical chemistry

Parallel determination of auxin and cytokinin levels within plant organs and tissues represents an invaluable tool for studies of their physiological effects and mutual interactions. Thanks to their different chemical structures, auxins, cytokinins and their metabolites are often determined separately, using specialized procedures of sample purification, extraction, and quantification. However, recent progress in the sensitivity of analytical methods of liquid chromatography coupled to mass spectrometry (LC-MS) allows parallel analysis of multiple compounds. Here we describe a method that is based on single step purification protocol followed by LC-MS separation and detection for parallel analysis of auxins, cytokinins and their metabolites in various plant tissues and cell cultures.
Permanent Link: http://hdl.handle.net/11104/0276151