X-ray-based microstructural analysis of hydrogel-based composites for bone tissue engineering

0480347 - ÚTAM 2018 RIV SI eng A - Abstract
Kytýř, Daniel - Krčmářová, Nela - Doktor, Tomáš - Kumpová, Ivana - Koudelková, Veronika - Nepomucká, Kateřina - Šepitka, J. - Gantar, A.
X-ray-based microstructural analysis of hydrogel-based composites for bone tissue engineering.
25. Mednarodna konferenca o materialih in tehnologijah. Program in knjiga povzetkov. Ljubljana: Inštitut za kovinske materiale in tehnologije, 2017 - (Godec, M.; Donik, Č.; Hočevar, M.; Kocijan, A.). s. 114-114. ISBN 978-961-94088-1-0.
[Mednarodna konferenca o materialih in tehnologijah /25./. 16.10.2017-19.10.2017, Portorož]
EU Projects: European Commission(XE) ATCZ133
Institutional support: RVO:68378297 ; RVO:68378050
Keywords : gellan gum * bioactive glass * artificial bone structures
OECD category: Composites (including laminates, reinforced plastics, cermets, combined natural and synthetic fibre fabrics
http://icmt25.com/e_files/content/Preliminary_BookOfAbstracts_25ICM&T.pdf

Artificial bone structures implantation becomes a promising treatment procedure in regenerative medicine. To ensure proper function of the scaffold its structural, mechanical and permeability properties have to be investigated and optimized. This study is focused on biodegradable hydrogel gellan gum (GG) reinforced by bioactive glass (BAG) nanoparticles with 0, 50 and 70 wt.%. The samples were subjected to tomografic imaging to obtain detail information about the internal microstructure and BAG particles distribution. As a comparative measurement planar energydispersive X-ray spectroscopy using scanning electron microscope and nanoindentation measurement were performed. The microtomography inspection was performed using the patented (European patent no. EP2835631) in-house designed modular radiographic imaging device equipped with scintillators, large single photon counting and spectroscopic detectors. From reconstructed volumetric data internal microarchitecture, porosity, cell-wall thickness and BAG distribution were derived. The results were compared with the results of the planar analysis of thin scaffold layers prepared by cryosection.
Permanent Link: http://hdl.handle.net/11104/0276141