Morphological characteristics of colitis developing in SCID mice reconstituted with CD4+CD45RBhigh T cells and influence of intestinal bacteria

0479935 - MBÚ 2018 eng A - Abstract
Štěpánková, Renata - Powrie, F. - Kofroňová, Olga - Kozáková, Hana - Hudcovic, Tomáš - Hrnčíř, Tomáš - Tlaskalová-Hogenová, Helena
Morphological characteristics of colitis developing in SCID mice reconstituted with CD4+CD45RBhigh T cells and influence of intestinal bacteria.
Food, Microbiota and Immunity „For strong immunity – feed your microbiota well“. Praha: Czech immunological society, 2017. s. 57-57.
[Food, Microbiota and Immunity. 07.06.2017-10.06.2017, Třešť]
R&D Projects: GA ČR(CZ) GA15-09518S; GA ČR(CZ) GA17-07332S
Institutional support: RVO:61388971
Keywords : Germfree * colitis * T cells
OECD category: Immunology

The subpopulation of T cells CD4+CD45RBhigh was purified from spleen of conventional BALB/c mice by magnetic separation (MACS) and cells were transferred. i.p. into immunodeficient SCID mice. Recipient germfree SCID mice were colonized by defined cocktail of bacteria isolated from SPF (Specific Pathogen Free) mice (Oxford Laboratory). Other recipient SCID mice were monoassociated with SFB (Segmented Filamentous Bacteria) bacteria; control SCID mice were either germfree or conventional. 8-12 weeks after the cell transfer morphological changes in colon and terminal ileum were evaluated by histology (H&E, PAS-Schiff) and scanning electron microscopy. Immunohistological analysis of expression of α-defensin and tight junctions (TJs) structural proteins (ZO-1 and Claudin -1) on ileal mucosa was performed. Colitis was present only in SCID recipients colonized with defined SPF cocktail microflora plus SFB bacteria. We found decreased production of mucus by goblet cells as an important feature of intestinal inflammation.
Permanent Link: http://hdl.handle.net/11104/0275850