Bioremediation of long-term PCB-contaminated soil by white-rot fungi

0479709 - MBÚ 2018 RIV NL eng J - Journal Article
Stella, Tatiana - Covino, Stefano - Čvančarová, Monika - Filipová, Alena - Petruccioli, M. - D´Annibale, A. - Cajthaml, Tomáš
Bioremediation of long-term PCB-contaminated soil by white-rot fungi.
Journal of Hazardous Materials. Roč. 324, FEB 15 PART B (2017), s. 701-710. ISSN 0304-3894. E-ISSN 1873-3336
R&D Projects: GA TA ČR TE01020218; GA ČR(CZ) GA15-02328S
Institutional support: RVO:61388971
Keywords : Pleurotus ostreatus * Ligninolytic fungi * Polychlorinated biphenyls
OECD category: Microbiology
Impact factor: 6.434, year: 2017

The objective of this work was to test the PCB-degrading abilities of two white-rot fungi, namely Pleurotus ostreatus and Irpex lacteus, in real contaminated soils with different chemical properties and autochthonous microflora. In addition to the efficiency in PCB removal, attention was given to other important parameters, such as changes in the toxicity and formation of PCB transformation products. Moreover, structural shifts and dynamics of both bacterial and fungal communities were monitored using next-generation sequencing and phospholipid fatty acid analysis. The best results were obtained with P. ostreatus, which resulted in PCB removals of 18.5, 41.3 and 50.5% from the bulk, top (surface) and rhizosphere, respectively, of dumpsite soils after 12 weeks of treatment. Numerous transformation products were detected (hydoxylated and methoxylated PCBs, chlorobenzoates and chlorobenzyl alcohols), which indicates that both fungi were able to oxidize and decompose the aromatic moiety of PCBs in the soils. Microbial community analysis revealed that P. ostreatus efficiently colonized the soil samples and suppressed other fungal genera. However, the same fungus substantially stimulated bacterial taxa that encompass putative PCB degraders. The results of this study finally demonstrated the feasibility of using this fungus for possible scaled-up bioremediation applications.
Permanent Link: http://hdl.handle.net/11104/0275671