Combination of RT-PCR and proteomics for the identification of Crimean-Congo hemorrhagic fever virus in ticks

0479555 - BC 2018 RIV GB eng J - Journal Article
Fernández de Mera, I.G. - Chaligiannis, I. - Hernández-Jarguín, A. - Villar, M. - Mateos-Hernández, L. - Papa, A. - Sotiraki, S. - Ruiz-Fons, F. - Cabezas Cruz, Alejandro - Gortázar, C. - de la Fuente, J.
Combination of RT-PCR and proteomics for the identification of Crimean-Congo hemorrhagic fever virus in ticks.
Heliyon. Roč. 3, July (2017), č. článku e00353. ISSN 2405-8440. E-ISSN 2405-8440
EU Projects: European Commission(XE) 278976 - ANTIGONE
Institutional support: RVO:60077344
Keywords : evolution * genetics * infectious disease * public health * veterinary science * virology
OECD category: Biochemistry and molecular biology
Method of publishing: Open access
https://www.cell.com/heliyon/pdf/S2405-8440(17)31474-3.pdf

Crimean-Congo hemorrhagic fever (CCHF) is an emerging tick-borne zoonotic disease caused by the CCHF virus (CCHFV). In this study, an experimental approach combining RT-PCR and proteomics was used for the identification and characterization of CCHFV in 106 ticks from 7 species that were collected from small ruminants in Greece. The methodological approach included an initial screening for CCHFV by RT-PCR followed by proteomics analysis of positive and control negative tick samples. This novel approach allowed the identification of CCHFV-positive ticks and provided additional information to corroborate the RT-PCR findings using a different approach. Two ticks, Dermacentor marginatus and Haemaphysalis parva collected from a goat and a sheep, respectively were positive for CCHFV. The sequences for CCHFV RNA segments S and L were characterized by RT-PCR and proteomics analysis of tick samples, respectively. These results showed the possibility of combining analyses at the RNA and protein levels using RT-PCR and proteomics for the characterizatio n of CCHFV in ticks. The results supported that the CCHFV identified in ticks are genetic variants of the AP92 strain. Although the AP92-like strains probably do not represent a high risk of CCHF to the population, the circulation of genetically diverse CCHFV strains could potentially result in the appearance of novel viral genotypes with increased pathogenicity and fitness.
Permanent Link: http://hdl.handle.net/11104/0275537