Analysis of European honeybee (Apis mellifera) wings using ATR-FTIR and Raman spectroscopy: A pilot study

0479457 - ÚSMH 2018 RIV CZ eng J - Journal Article
Machovič, Vladimír - Lapčák, L. - Havelcová, Martina - Borecká, Lenka - Novotná, M. - Novotná, M. - Javůrková, I. - Langrová, I. - Hájková, Š. - Brožová, A. - Titěra, D.
Analysis of European honeybee (Apis mellifera) wings using ATR-FTIR and Raman spectroscopy: A pilot study.
Scientia Agriculturae Bohemica. Roč. 48, č. 1 (2017), s. 22-29. ISSN 1211-3174. E-ISSN 1805-9430
Institutional support: RVO:67985891
Keywords : honeybee wings * ATR-FTIR * Raman spectroscopy * protein * lipid * chitin
OECD category: Analytical chemistry

The infrared (ATR-FTIR) and Raman spectroscopy was used for the structural characterization of honeybee wings. Protein, chitin, and lipid characteristic spectral features were detected using both methods. The protein secondary structure was predominantly composed of the beta-sheet molecular conformation with beta-turns or coil contributions. The vibration modes of the side-chain aromatic amino acid residues (tyrosine, phenylalanine, tryptophan) occurred in the wing spectra. The results of discriminant analysis showed that the infrared spectroscopy of the wing in combination with a multivariate analysis seemed promising for a resolution of the chemical structure of the wings based on lipid, proteins, and chitin content.
Permanent Link: http://hdl.handle.net/11104/0275493