Molecular analyses reveal high species diversity of trematodes in a sub-Arctic lake

0479250 - BC 2018 RIV AU eng J - Journal Article
Soldánová, Miroslava - Georgieva, Simona - Roháčová, Jana - Knudsen, R. - Kuhn, J. A. - Henriksen, E. H. - Siwertsson, A. - Shaw, J. C. - Kuris, A. M. - Amundsen, P.-A. - Scholz, Tomáš - Lafferty, K. D. - Kostadinova, Aneta
Molecular analyses reveal high species diversity of trematodes in a sub-Arctic lake.
International Journal for Parasitology. Roč. 47, č. 6 (2017), s. 327-345. ISSN 0020-7519. E-ISSN 1879-0135
R&D Projects: GA ČR GA15-14198S; GA ČR GAP505/10/1562
Institutional support: RVO:60077344
Keywords : Trematode diversity * intermediate hosts * phylogeny * mitochondrial DNA * nuclear DNA * Lake Takvatn * Norway * Sub-Arctic
OECD category: Zoology
Impact factor: 3.078, year: 2017

To identify trematode diversity and life-cycles in the sub-Arctic Lake Takvatn, Norway, we characterised 120 trematode isolates from mollusc first intermediate hosts, metacercariae from second intermediate host fishes and invertebrates, and adults from fish and invertebrate definitive hosts, using molecular techniques. Phylogenies based on nuclear and/or mtDNA revealed high species richness (24 species or species-level genetic lineages) and uncovered trematode diversity (16 putative new species) from five families typical in lake ecosystems (Allocreadiidae, Diplostomidae, Plagiorchiidae, Schistosomatidae and Strigeidae). Sampling potential invertebrate hosts allowed matching of sequence data for different stages, thus achieving molecular elucidation of trematode life-cycles and exploration of host-parasite interactions. Phylogenetic analyses also helped identify three major mollusc intermediate hosts (Radix balthica, Pisidium casertanum and Sphaerium sp.) in the lake. Our findings increase the known trematode diversity at the sub-Arctic Lake Takvatn, showing that digenean diversity is high in this otherwise depauperate sub-Arctic freshwater ecosystem and indicating that sub-Arctic and Arctic ecosystems may be characterised by unique trematode assemblages. (C) 2017 Australian Society for Parasitology. Published by Elsevier Ltd. All rights reserved.
Permanent Link: http://hdl.handle.net/11104/0275244