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Physical map of the short arm of bread wheat chromosome 3D

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    0479190 - ÚEB 2018 RIV US eng J - Journal Article
    Holušová, Kateřina - Vrána, Jan - Šafář, Jan - Šimková, Hana - Balcárková, Barbora - Frenkel, Z. - Darrier, B. - Paux, E. - Cattonaro, F. - Berges, H. - Letellier, T. - Alaux, M. - Doležel, Jaroslav - Bartoš, Jan
    Physical map of the short arm of bread wheat chromosome 3D.
    Plant Genome. Roč. 10, č. 2 (2017). E-ISSN 1940-3372
    R&D Projects: GA ČR GA13-08786S; GA MŠk(CZ) LO1204
    Institutional support: RVO:61389030
    OECD category: Plant sciences, botany
    Impact factor: 2.923, year: 2017

    Bread wheat (Triticum aestivum L.) is one of the most important crops worldwide. Although a reference genome sequence would represent a valuable resource for wheat improvement through genomics-assisted breeding and gene cloning, its generation has long been hampered by its allohexaploidy, high repeat content, and large size. As a part of a project coordinated by the International Wheat Genome Sequencing Consortium (IWGSC), a physical map of the short arm of wheat chromosome 3D (3DS) was prepared to facilitate reference genome assembly and positional gene cloning. It comprises 869 contigs with a cumulative length of 274.5 Mbp and represents 85.5% of the estimated chromosome arm size. Eighty-six Mbp of survey sequences from chromosome arm 3DS were assigned in silico to physical map contigs via next-generation sequencing of bacterial artificial chromosome pools, thus providing a high-density framework for physical map ordering along the chromosome arm. About 60% of the physical map was anchored in this single experiment. Finally, 1393 high-confidence genes were anchored to the physical map. Comparisons of gene space of the chromosome arm 3DS with genomes of closely related species [Brachypodium distachyon (L.) P.Beauv., rice (Oryza sativa L.), and sorghum [Sorghum bicolor (L.) Moench] and homeologous wheat chromosomes provided information about gene movement on the chromosome arm.
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    2017_Holusova_PLANT GENOME_.pdf11.4 MBOtheropen-access
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