Low-thrombogenic fibrin-heparin coating promotes in vitro endothelialization

0478912 - ÚMCH 2018 RIV US eng J - Journal Article
Kaplan, Ondřej - Hierlemann, T. - Krajewski, S. - Kurz, J. - Nevoralová, Martina - Houska, Milan - Riedel, Tomáš - Riedelová, Zuzana - Zárubová, Jana - Wendel, H. P. - Brynda, Eduard
Low-thrombogenic fibrin-heparin coating promotes in vitro endothelialization.
Journal of Biomedical Materials Research. Part A. Roč. 105, č. 11 (2017), s. 2995-3005. ISSN 1549-3296. E-ISSN 1552-4965
R&D Projects: GA MZd(CZ) NV15-29153A
Institutional support: RVO:61389013 ; RVO:67985823
Keywords : fibrin-heparin coating * hemocompatibility * endothelialization
OECD category: Cardiac and Cardiovascular systems; Cardiac and Cardiovascular systems (FGU-C)
Impact factor: 3.231, year: 2017

Long-term performance of implanted cardiovascular grafts can be ensured if living endothelium overgrows their surface. Surface modifications to implants are therefore being sought that can encourage endothelialization while preventing thrombus formation until the natural endothelium is formed. In the present study, heparin was covalently attached to a fibrin mesh grown from a polyvinyl chloride (PVC) substrate surface by the catalytic action of surface immobilized thrombin on a fibrinogen solution. The coating prevented platelet activation, thrombin generation and clot formation, and reduced inflammatory reactions when exposed to fresh human whole blood circulating in a Chandler loop model. In addition, in vitro seeded human umbilical vein and human saphenous vein endothelial cells showed considerably enhanced attachment and proliferation on the coating.
Permanent Link: http://hdl.handle.net/11104/0276410