0478906 - ÚEM 2018 RIV GB eng J - Journal Article
Kubinová, Šárka - Závišková, Kristýna - Uherková, Lenka - Zablotskyy, Vitaliy A. - Churpita, Olexandr - Lunov, Oleg - Dejneka, Alexandr
Non-thermal air plasma promotes the healing of acute skin wounds in rats.
Scientific Reports. Roč. 7, mar (2017), s. 45183. ISSN 2045-2322. E-ISSN 2045-2322
R&D Projects: GA MŠMT(CZ) LO1309; GA MŠMT(CZ) LM2015064; GA TA ČR(CZ) TA04010449
Institutional support: RVO:68378041 ; RVO:68378271
Keywords : randomized controlled-trial * atmospheric argon plasma * nitric-oxide
OECD category: Biochemical research methods; Fluids and plasma physics (including surface physics) (FZU-D)
Impact factor: 4.122, year: 2017 ; AIS: 1.356, rok: 2017
DOI: https://doi.org/10.1038/srep45183

Non-thermal plasma (NTP) has nonspecific antibacterial effects, and can be applied as an effective tool for the treatment of chronic wounds and other skin pathologies. In this study we analysed the effect of NTP on the healing of the full-thickness acute skin wound model in rats. We utilised a single jet NTP system generating atmospheric pressure air plasma, with ion volume density 5.10(17) m(-3) and gas temperature 30-35 degrees C. The skin wounds were exposed to three daily plasma treatments for 1 or 2 minutes and were evaluated 3, 7 and 14 days after the wounding by histological and gene expression analysis. NTP treatment significantly enhanced epithelization and wound contraction on day 7 when compared to the untreated wounds. Macrophage infiltration into the wound area was not affected by the NTP treatment. Gene expression analysis did not indicate an increased inflammatory reaction or a disruption of the wound healing proces, transient enhancement of inflammatory marker upregulation was found after NTP treatment on day 7. In summary, NTP treatment had improved the healing efficacy of acute skin wounds without noticeable side effects and concomitant activation of pro-inflammatory signalling. The obtained results highlight the favourability of plasma applications for wound therapy in clinics.
Permanent Link: http://hdl.handle.net/11104/0274959