Characterization of Two Historic Smallpox Specimens from a Czech Museum

0478813 - ÚMG 2018 RIV CH eng J - Journal Article
Pajer, P. - Dresler, J. - Kabickova, H. - Píša, L. - Aganov, P. - Fucik, K. - Elleder, Daniel - Hron, Tomáš - Kuželka, V. - Velemínský, P. - Klimentová, J. - Fučíková, A. - Pejchal, J. - Hrabáková, Rita - Beneš, V. - Rausch, T. - Dundr, P. - Pilin, A. - Čabala, R. - Hubálek, Martin - Stříbrný, J. - Antwerpen, M.H. - Meyer, H.
Characterization of Two Historic Smallpox Specimens from a Czech Museum.
Viruses. Roč. 9, č. 8 (2017), č. článku 200. E-ISSN 1999-4915
Institutional support: RVO:68378050 ; RVO:67985904 ; RVO:61388963
Keywords : smallpox * variola virus * evolution * next generation suquencing * historic specimen * phylogeny
OECD category: Virology; Virology (UZFG-Y); Virology (UOCHB-X)
Impact factor: 3.761, year: 2017

Although smallpox has been known for centuries, the oldest available variola virus strains were isolated in the early 1940s. At that time, large regions of the world were already smallpox-free. Therefore, genetic information of these strains can represent only the very last fraction of a long evolutionary process. Based on the genomes of 48 strains, two clades are differentiated: Clade 1 includes variants of variola major, and clade 2 includesWest African and variola minor (Alastrim) strains. Recently, the genome of an almost 400-year-old Lithuanian mummy was determined, which fell basal to all currently sequenced strains of variola virus on phylogenetic trees. Here, we determined two complete variola virus genomes from human tissues kept in a museum in Prague dating back 60 and 160 years, respectively. Moreover, mass spectrometry-based proteomic, chemical, and microscopic examinations were performed. The 60-year-old specimen was most likely an importation from India, a country with endemic smallpox at that time. The genome of the 160-year-old specimen is related to clade 2 West African and variola minor strains. This sequence likely represents a new endemic European variant of variola virus circulating in the midst of the 19th century in Europe.
Permanent Link: http://hdl.handle.net/11104/0274867