Real-time PCR quantification of arbuscular mycorrhizal fungi: does the use of nuclear or mitochondrial markers make a difference?

0478214 - BÚ 2018 RIV DE eng J - Journal Article
Voříšková, Alena - Jansa, J. - Püschel, David - Krüger, Manuela - Cajthaml, T. - Vosátka, Miroslav - Janoušková, Martina
Real-time PCR quantification of arbuscular mycorrhizal fungi: does the use of nuclear or mitochondrial markers make a difference?
Mycorrhiza. Roč. 27, č. 6 (2017), s. 577-585. ISSN 0940-6360. E-ISSN 1432-1890
R&D Projects: GA ČR GA15-05466S
Institutional support: RVO:67985939
Keywords : real-time PCR * quantification * arbuscular mycorrhizal fungi
OECD category: Plant sciences, botany
Impact factor: 2.778, year: 2017

Copy numbers of nuclear ribosomal DNA (nrDNA) and mitochondrial DNA (mtDNA) were closely related within all isolates, however, the slopes and intercepts of the linear relationships significantly differed among the isolates. Across all isolates, a large proportion of variance in nrDNA copy numbers was explained by root colonization intensity or contents of AMF-specific PLFA, while variance in mtDNA copy numbers was mainly explained by differences among AMF isolates. Our results suggest that nrDNA is a more suitable marker region than mtDNA for the quantification of multiple AMF taxa as its copy numbers are better related to fungal biomass across taxa than are copy numbers of mtDNA.
Permanent Link: http://hdl.handle.net/11104/0277646