Separation of oligopeptides, nucleobases, nucleosides and nucleotides using capillary electrophoresis/electrochromatography with sol–gel modified inner capillary wall

0478035 - FGÚ 2018 RIV NL eng J - Journal Article
Svobodová, Jana - Kofroňová, Olga - Benada, Oldřich - Král, V. - Mikšík, Ivan
Separation of oligopeptides, nucleobases, nucleosides and nucleotides using capillary electrophoresis/electrochromatography with sol–gel modified inner capillary wall.
Journal of Chromatography A. Roč. 1517, Sep 29 (2017), s. 185-194. ISSN 0021-9673. E-ISSN 1873-3778
R&D Projects: GA ČR(CZ) GA15-01948S; GA MŠMT(CZ) LO1509
Institutional support: RVO:67985823 ; RVO:61388971
Keywords : capillary electrochromatography (CEC) * open-tubular capillary electrochromatography (OT-CEC) * nucleo-compounds * oligopeptides * sol–gel methods * Porphyrin * scanning electron microscopy (SEM)
OECD category: Analytical chemistry; Biochemistry and molecular biology (MBU-M)
Impact factor: 3.716, year: 2017

The aim of this article is to study the modification of an inner capillary wall with sol–gel coating (pure silica sol–gel or silica sol–gel containing porphyrin-brucine conjugate) and determine its influence on the separation process using capillary electrophoresis/electrochromatography method. After modification of the inner capillary surface the separation of analytes was performed using two different phosphate buffers (pH 2.5 and 9.0) and finally the changes in electrophoretic mobilities of various samples were calculated. To confirm that the modification of the inner capillary surface was successful, the parts of the inner surfaces of capillaries were observed using scanning electron microscopy. The analytes used as testing samples were oligopeptides, nucleosides, nucleobases and finally nucleotides.
Permanent Link: http://hdl.handle.net/11104/0274264