Cytokinin metabolism of pathogenic fungus Leptosphaeria maculans involves isopentenyltransferase, adenosine kinase and cytokinin oxidase/dehydrogenase

0477225 - ÚEB 2018 RIV CH eng J - Journal Article
Trdá, Lucie - Barešová, Monika - Šašek, Vladimír - Nováková, Miroslava - Zahajská, Lenka - Dobrev, Petre - Motyka, Václav - Burketová, Lenka
Cytokinin metabolism of pathogenic fungus Leptosphaeria maculans involves isopentenyltransferase, adenosine kinase and cytokinin oxidase/dehydrogenase.
Frontiers in Microbiology. Roč. 8, JUL 21 (2017), č. článku 1374. ISSN 1664-302X. E-ISSN 1664-302X
R&D Projects: GA ČR GA13-26798S; GA ČR(CZ) GA16-14649S
Institutional support: RVO:61389030
Keywords : Adenosine kinase * Cytokinin * Cytokinin oxidase/dehydrogenase * Isopentenyltransferase * Leptosphaeria maculans * Zeatin cis/trans isomerase
OECD category: Microbiology
Impact factor: 4.019, year: 2017

Among phytohormones, cytokinins (CKs) play an important role in controlling crucial aspects of plant development. Not only plants but also diverse microorganisms are able to produce phytohormones, including CKs, though knowledge concerning their biosynthesis and metabolism is still limited. In this work we demonstrate that the fungus Leptosphaeria maculans, a hemi-biotrophic pathogen of oilseed rape (Brassica napus), causing one of the most damaging diseases of this crop, is able to modify the CK profile in infected B. napus tissues, as well as produce a wide range of CKs in vitro, with the cis-zeatin derivatives predominating. The endogenous CK spectrum of L. maculans in vitro consists mainly of free CK bases, as opposed to plants, where other CK forms are mostly more abundant. Using functional genomics, enzymatic and feeding assays with CK bases supplied to culture media, we show that L. maculans contains a functional: (i) isopentenyltransferase (IPT) involved in cZ production, (ii) adenosine kinase (AK) involved in phosphorylation of CK ribosides to nucleotides, and (iii) CK-degradation enzyme cytokinin oxidase/dehydrogenase (CKX). Our data further indicate the presence of cis-trans isomerase, zeatin O-glucosyltransferase(s) and N 6(Δ 2isopentenyl)adenine hydroxylating enzyme. Besides, we report on a crucial role of LmAK for L. maculans fitness and virulence. Altogether, in this study we characterize in detail the CK metabolism of the filamentous fungi L. maculans and report its two novel components, the CKX and CK-related AK activities, according to our knowledge for the first time in the fungal kingdom. Based on these findings, we propose a model illustrating CK metabolism pathways in L. maculans.
Permanent Link: http://hdl.handle.net/11104/0273617