Interaction of Newly Platinum(II) with Tris(2-carboxyethyl)phosphine Complex with DNA and Model Lipid Membrane

0476865 - ÚFCH JH 2018 RIV US eng J - Journal Article
Pruchnik, H. - Kral, Teresa - Hof, Martin
Interaction of Newly Platinum(II) with Tris(2-carboxyethyl)phosphine Complex with DNA and Model Lipid Membrane.
Journal of Membrane Biology. Roč. 250, č. 5 (2017), s. 461-470. ISSN 0022-2631. E-ISSN 1432-1424
R&D Projects: GA ČR(CZ) GBP208/12/G016
Institutional support: RVO:61388955
Keywords : dna * DPPC bilayer * dsc * IR spectroscopy * Platinum(II) complex * tcspc-fcs
OECD category: Physical chemistry
Impact factor: 1.638, year: 2017
Method of publishing: Open access

Structural properties of plasmid DNA and model lipid membrane treated with newly synthesized platinum(II) complex cis-[PtCl 2 {P(CH 2 CH 2 COOH) 3 } 2 ] (cis-DTCEP for short) were studied and compared with effects of anticancer drug cisplatin, cis-[Pt(NH 3 ) 2 Cl 2 ] (cis-DDP for short). Time Correlated Single Photon Counting Fluorescence Correlation Spectroscopy (TCSPC-FCS) was employed to study interactions between those platinum complexes and DNA. The TCSPC-FCS results suggest that bonding of cis-DTCEP derivative to DNA leads to plasmid strain realignment towards much more compact structure than in the case of cis-DDP. Application of both differential scanning calorimetry and infrared spectroscopy to platinum complexes/DPPC showed that cis-DTCEP slightly increases the phospholipid’s main phase transition temperature resulting in decreased fluidity of the model membrane. The newly investigated compound-similarly to cis-DDP-interacts mainly with the DPPC head group however not only by the means of electrostatic forces: this compound probably enters into hydrophilic region of the lipid bilayer and forms hydrogen bonds with COO groups of glycerol and PO 2 - group of DPPC.
Permanent Link: http://hdl.handle.net/11104/0273283