Chromosome identification for the carnivorous plant Genlisea margaretae

0476530 - ÚEB 2018 RIV DE eng J - Journal Article
Tran, T.D. - Šimková, Hana - Schmidt, R. - Doležel, Jaroslav - Schubert, I. - Fuchs, J.
Chromosome identification for the carnivorous plant Genlisea margaretae.
Chromosoma. Roč. 126, č. 3 (2017), s. 389-397. ISSN 0009-5915. E-ISSN 1432-0886
R&D Projects: GA MŠMT(CZ) LO1204
Institutional support: RVO:61389030
Keywords : BACs * Genlisea * Karyotyping * Multicolor fluorescence in situ hybridization (mcFISH) * Reprobing
OECD category: Plant sciences, botany
Impact factor: 4.021, year: 2017

Genlisea margaretae, subgenus Genlisea, section Recurvatae (184 Mbp/1C), belongs to a plant genus with a 25-fold genome size difference and an extreme genome plasticity. Its 19 chromosome pairs could be distinguished individually by an approach combining optimized probe pooling and consecutive rounds of multicolor fluorescence in situ hybridization (mcFISH) with bacterial artificial chromosomes (BACs) selected for repeat-free inserts. Fifty-one BACs were assigned to 18 chromosome pairs. They provide a tool for future assignment of genomic sequence contigs to distinct chromosomes as well as for identification of homeologous chromosome regions in other species of the carnivorous Lentibulariaceae family, and potentially of chromosome rearrangements, in cases where more than one BAC per chromosome pair was identified.
Permanent Link: http://hdl.handle.net/11104/0273009