Natural compounds and their effect on 5-fluorouracil in colorectal cancer cell lines

0475790 - ÚEM 2017 RIV CZ eng C - Conference Paper (international conference)
Čumová, Andrea - Opattová, Alena - Vodenková, Soňa - Horák, Josef - Slíva, D. - Vodička, Pavel
Natural compounds and their effect on 5-fluorouracil in colorectal cancer cell lines.
Program a abstrakta. Genetická toxikologie a prevence rakoviny. Zlín: Alisa Group, 2016, s. 8-8. ISBN 978-80-88038-04-7.
[Genetická toxikologie a prevence rakoviny. Telč (CZ), 11.05.2016-13.05.2016]
R&D Projects: GA MŠMT(CZ) LH13061; GA MZd(CZ) NV15-27580A
Institutional support: RVO:68378041
Keywords : colorectal cancer * natural compound * p53 * DNA repair * 5-fluorouracil * drug resistance
Subject RIV: EE - Microbiology, Virology

Colorectal cancer (CRC) is the second most common type of cancer and the second most common cause of cancer related deaths in Europe. 5-Fluorouracil (5-FU) is widely used in treatment of various cancers including CRC, but apart from the cytotoxic effect on cancer cells may also cause adverse toxic side effects. 5-FU is an anti-metabolite with chemical structure similar to that of the pyrimidine molecules of DNA and RNA. However, response to chemotherapy is often limited by drug resistance. The p53 protein is one of the most widely studied tumour suppressors and mutations in TP53 gene are frequently detected in different types of tumours.
Ganoderma Lucidum (GLC) is a mushroom used in Traditional Eastern Medicine which exhibits anti-cancer and anti-proliferative effects in vitro
The aim of our study is to define the role of p53 in the interaction between 5-FU and GLC extract and their simultaneous effect on survival in CRC cell lines.
Our results suggest that GLC extract significantly increases cytotoxicity and genotoxicity of 5-FU in CRC lines with different p53 status and may potentially modulate the response of p53 knock-out cells which are less sensitive to 5-FU treatment. Interaction of conventional chemotherapeutics with natural compounds introduces a novel aspect in cancer research and therapy.


Permanent Link: http://hdl.handle.net/11104/0272424