Detection of G-protein coupled estrogen receptor (GPER) in the male reproductive tissue and spermatic cells during their development and maturation in pigs.\n\n

0474844 - BTÚ 2018 CZ eng A - Abstract
Krejčířová, R. - Sommerová, V. - Rajmon, R. - Postlerová, Pavla
Detection of G-protein coupled estrogen receptor (GPER) in the male reproductive tissue and spermatic cells during their development and maturation in pigs.

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Book of abstracts XXIIIrd Symposium of Immunology and Biology of Reproduction. Vestec u Prahy: Biotechnologický ústav AVČR v. v. i., 2017 - (Kubátová, a.). s. 26-26
[XXIIIrd Symposium of Immunology and Biology of Reproduction. 18.05.2017-20.05.2017, Třešť]
R&D Projects: GA MŠMT(CZ) ED1.1.00/02.0109
Institutional support: RVO:86652036
Keywords : estrogens * estrogen receptors * GPER * spermatozoa * sperm capacitation * sperm maturation
OECD category: Biochemical research methods

Effect of estrogenic substances on the male reproductive tissue and development of gametes is an essential for the reproductive success. Estrogens affect the target cells via estrogen receptors (ERs) by both genomic and non-genomic pathways. The G-protein coupled estrogen receptor (GPER) is a transmembrane receptor mediating rapid non-genomic responses of estrogens. ERs have been found in the male reproductive tract in many mammalian species including humans. GPER has not been detected in reproductive tissues of pigs yet. Its presence has been only described in boar ejaculated spermatozoa (Rago et al., J Anat 2014;224(6):732-6). We detected GPER in testicular and epididymal tissues and in epididymal spermatozoa using specific antibody G-19 by immunofluorescent microscopy. In the testis GPER was found in Leydig and spermatic cells. In epididymis, we immunolocalized GPER in plasma membrane of the secretory epithelium and in spermatozoa at the lumen of cauda epididymis tubule. In spermatozoa isolated from the epididymis GPER was localized in the acrosome and flagellum. Our results confirmed the presence GPER in boar epididymal sperm for the first time. The localization of GPER in the plasma membrane of epididymal secretory tissue and spermatozoa suggests the possible involvement of estrogens not only in the sperm development, but in the sperm post-testicular maturation.







Permanent Link: http://hdl.handle.net/11104/0273886