The use of histological methods to detect testicular pathology.\n\n

0474840 - BTÚ 2018 CZ eng A - Abstract
Margaryan, Hasmik - Pavlínková, Gabriela - Bohuslavová, Romana - Dvořáková-Hortová, Kateřina - Pěknicová, Jana
The use of histological methods to detect testicular pathology.

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Book of abstracts XXIIIrd Symposium of Immunology and Biology of Reproduction. Vestec u Prahy: Biotechnologický ústav AVČR v. v. i., 2017 - (Kubátová, a.). s. 21-22
[XXIIIrd Symposium of Immunology and Biology of Reproduction. 18.05.2017-20.05.2017, Třešť]
R&D Projects: GA MŠMT(CZ) ED1.1.00/02.0109; GA MZd(CZ) AZV 15-30880A
Institutional support: RVO:86652036
Keywords : diabetes mellitus * monoclonal antibody * spermatogenesis * mitosis * meiosis * seminiferous tubules
OECD category: Biochemistry and molecular biology

Histological analysis of the testes may help to determine the scope of tissue damage under the influence of various substances (endocrine disruptors) as well as metabolic diseases (diabetes mellitus). Using hematoxylin & eosin stained sections, the morphometric evaluations of seminiferous tubules can be performed, particularly the tubule diameter and thickness of the seminiferous epithelium.
In our case, we used a panel of antibodies and mouse model to evaluate changes in the testicular tissue affected by metabolic disease - diabetes mellitus.
Selected antibodies against different proteins were used for detection of germ cells during spermatogenesis, mitotic and meiotic division, tissue architecture and other parameters.
The histology of control testes showed smooth rounded seminiferous tubules flanked by embryonic epithelium at various stages of spermatogenesis. In contrast, in the testes of diabetic mice, thinner disrupted germinal epithelium was noticeable, although all the distinct developmental stages of spermatogenesis, including spermatozoa in the lumen of the seminiferous tubules were identified. We observed high individual variations in testicular histology within the diabetic group. Some seminiferous tubules showed the early spermatocytes and spermatids, which did not form regular "columns". Premature spermatocytes or some seminiferous tubules were anthropic without any cells in the tubule lumen.
The panel of antibodies can be used to test differences in healthy and damaged organs in other mammals, including human.






Permanent Link: http://hdl.handle.net/11104/0273889