Comparative study of tetraspanins on porcine and bovine oocytes.\n

0474834 - BTÚ 2018 CZ eng A - Abstract
Antalíková, J. - Jankovičová, J. - Simon, M. - Šecová, P. - Michalková, K. - Horovská, L. - Chmelíková, E. - Postlerová, Pavla
Comparative study of tetraspanins on porcine and bovine oocytes.
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Book of abstracts XXIIIrd Symposium of Immunology and Biology of Reproduction. Vestec u Prahy: Biotechnologický ústav AVČR v. v. i., 2017 - (Kubátová, a.). s. 15-15
[XXIIIrd Symposium of Immunology and Biology of Reproduction. 18.05.2017-20.05.2017, Třešť]
R&D Projects: GA MŠMT(CZ) ED1.1.00/02.0109; GA AV ČR(CZ) SAV-AVČR 15-05
Institutional support: RVO:86652036
Keywords : tetraspanin * CD9 * CD81 * oocyte
OECD category: Biochemistry and molecular biology

Tetraspanins are multifunctional molecules located in specific microdomains on the plasma membrane. Thanks to their ability to enter in molecular partnerships with other members of tetraspanin family or other proteins they can form tetraspanin web and thereby affect many cellular functions. Well described involvement of tetraspanins in somatic cell immune response, cell migration, viral infections, metastasis formation etc., definitely led to suggestion of their participation in the similar processes occurring during gametes interaction. Up to date, although an extensive study proved the fact that tetraspanins CD9 and CD81 are really directly involved in gamete interaction of mammals, their precise role in the fertilization process is not clear yet. In the present study, we compared the localisation of these two tetraspanins on bovine and porcine oocytes in different stage of development. We examined the study of the possible role of CD9 and CD81 molecules in the fertilization process of cattle using polyclonal antibodies to CD9 and CD81 in in vitro fertilization assay. In our experiment, no significant reduction in a number of fertilized eggs and cleavage of zygotes after pre-treatment of oocytes with CD9 and CD81 antibodies were observed. The obtained results contribute to data in the scope of bovine and porcine species comparison in the field of fertilization process in mammals.
Permanent Link: http://hdl.handle.net/11104/0273894