Effect of crosslinking chemistry of albumin/heparin multilayers on FGF-2 adsorption and endothelial cell behavior

0473967 - ÚMCH 2018 RIV NL eng J - Journal Article
Kumorek, Marta M. - Janoušková, Olga - Höcherl, Anita - Houska, Milan - Mázl Chánová, Eliška - Kasoju, Naresh - Cuchalová, Lucie - Matějka, R. - Kubies, Dana
Effect of crosslinking chemistry of albumin/heparin multilayers on FGF-2 adsorption and endothelial cell behavior.
Applied Surface Science. Roč. 411, 31 July (2017), s. 240-250. ISSN 0169-4332. E-ISSN 1873-5584
R&D Projects: GA MŠMT(CZ) LQ1604; GA MŠMT(CZ) ED1.1.00/02.0109
Institutional support: RVO:61389013
Keywords : basic fibroblast growth factor * heparin * cross-linking
OECD category: Polymer science
Impact factor: 4.439, year: 2017

The biomaterials that efficiently deliver growth factors represent an important challenge in cell-based tissue engineering. Layer-by-layer (LbL) thin films are attractive for incorporating controlled amounts of growth factors and releasing them over time. Herein, we investigated the effect of a method of cross-linking of albumin/heparin layer-by-layer (LbL) assembly ((Alb/Hep)3) on the loading and release of basic fibroblast growth factor (FGF-2), and subsequent proliferation of human endothelial cells (HUVECs). The (Alb/Hep)3 assemblies were cross-linked using glutaraldehyde, reductive amination or carbodiimide chemistries, and then biofunctionalized with FGF-2. The (Alb/Hep)3 assemblies were characterized by the infrared multi-internal reflection spectroscopy, atomic force microscopy, ellipsometry, and surface plasmon resonance (SPR). The FGF-2 loading was quantified by the SPR in situ analysis. Our results showed that the (Alb/Hep)3 cross-linking affected the amount of the bound heparin (from 150 to 315 ng/cm2), amount of FGF-2 loaded (from 75 to 125 ng/cm2), FGF-2 release (from 15 to 53% over 8 days), and consequently the HUVEC cell proliferation (from 50 to 80 × 103 cells/cm2 at day 5). All FGF-2 loaded assemblies stimulated the cell growth more than a soluble FGF-2 added into the cell media. In particular, the highest HUVECs proliferation was detected on the carbodiimide-cross-linked assembly. Overall, these biocompatible cross-linked assemblies can fine-tune the loading and release of growth factor providing a platform for cell-contacting applications.
Permanent Link: http://hdl.handle.net/11104/0271129