Theoretical and experimental study of the antifreeze protein AFP752, trehalose and dimethyl sulfoxide cryoprotection mechanism: correlation with cryopreserved cell viability

0473751 - FZÚ 2018 RIV GB eng J - Journal Article
Kratochvílová, Irena - Golan, Martin - Pomeisl, Karel - Richter, Jan - Sedláková, Silvia - Šebera, Jakub - Mičová, Júlia - Falk, Martin - Falková, Iva - Řeha, David - Elliott, J.R. - Varga, K. - Follett, S.E. - Šimek, Daniel
Theoretical and experimental study of the antifreeze protein AFP752, trehalose and dimethyl sulfoxide cryoprotection mechanism: correlation with cryopreserved cell viability.
RSC Advances. Roč. 7, č. 1 (2017), s. 352-360. E-ISSN 2046-2069
R&D Projects: GA ČR(CZ) GA15-05095S; GA MŠMT LO1409; GA ČR(CZ) GA14-10279S; GA ČR(CZ) GA16-12454S; GA ČR GBP302/12/G157; GA MZd NV16-29835A
Grant - others:FUNBIO(XE) CZ.2.16/3.1.00/21568
Institutional support: RVO:68378271 ; RVO:61388971 ; RVO:68081707
Keywords : X-ray diffraction * DFT * Raman spectroscopy * DSC * cryoprotectants
OECD category: Biophysics
Impact factor: 2.936, year: 2017

The physico-chemical properties of selected cryoprotectants (antifreeze protein TrxA-AFP752, trehalose and dimethyl sulfoxide) were correlated with their impact on the constitution of ice and influence on frozen/thawed cell viability. The freezing processes and states of investigated materials solutions were described and explained from a fundamental point of view using ab initio modelling (molecular dynamics, DFT), Raman spectroscopy, differential scanning calorimetry and X-ray diffraction. For the first time, in this work we correlated the microscopic view (modelling) with the description of the frozen solution states and put these results in the context of human skin fibroblast viability after freezing and thawing. DMSO and AFP had different impacts on their solution's freezing process but in both cases the ice crystallinity size was considerably reduced. DMSO and AFP treatment in different ways improved the viability of frozen/thawed cells.
Permanent Link: http://hdl.handle.net/11104/0270892