An advanced conjugation strategy for the preparation of quantum dot-antibody immunoprobes

0473595 - ÚIACH 2018 RIV GB eng J - Journal Article
Dvořáková, V. - Čadková, M. - Datinská, Vladimíra - Klepárník, Karel - Foret, František - Bílková, Z. - Korecká, L.
An advanced conjugation strategy for the preparation of quantum dot-antibody immunoprobes.
Analytical Methods: advancing methods and applications. Roč. 9, č. 13 (2017), s. 1991-1997. ISSN 1759-9660. E-ISSN 1759-9679
R&D Projects: GA ČR(CZ) GA17-01995S
Institutional support: RVO:68081715
Keywords : quantum dots * immunoprobes * antibodies
OECD category: Analytical chemistry
Impact factor: 2.073, year: 2017

An advanced site-specific conjugation strategy for the preparation of quantum dot-based antibody probes applicable in various immunoassays from fluorescence to electrochemical biosensors is described. The combination of antigen (protein ApoE) modified magnetic particles providing protection to antibody binding sites, simple carbodiimide chemistry and carboxylate quantum dots (QDs) made of CdSe/ZnS is used for efficient labelling of anti-ApoE antibodies representing a model system. Polyacrylamide gel electrophoresis, fluorescence spectra measurements, capillary electrophoresis-laser induced fluorescence (CE-LIF) and square wave anodic stripping voltammetry (SWASV) were used for experimental verification of labelling efficiency. A simple change in antibody type makes this approach versatile and exploitable in a wide range of applications.
Permanent Link: http://hdl.handle.net/11104/0270725