Markers of Nucleic Acids and Proteins Oxidation among Office Workers Exposed to Air Pollutants Including (nano) TiO2 Particles.

0473541 - ÚCHP 2018 RIV SE eng J - Journal Article
Pelclová, D. - Ždímal, Vladimír - Kačer, P. - Vlčková, Š. - Fenclová, Z. - Navrátil, Tomáš - Komarc, M. - Schwarz, Jaroslav - Zíková, Naděžda - Makeš, Otakar - Zakharov, S.
Markers of Nucleic Acids and Proteins Oxidation among Office Workers Exposed to Air Pollutants Including (nano) TiO2 Particles.
Neuroendocrinology Letters. Roč. 37, Suppl.1 (2016), s. 13-16. ISSN 0172-780X. E-ISSN 2354-4716
Institutional support: RVO:67985858 ; RVO:61388955
Keywords : nanoparticles * exhaled breath condensate * oxidative stress * occupation * TiO
OECD category: Public and environmental health; Physical chemistry (UFCH-W)
Impact factor: 0.918, year: 2016

Experimental studies using nanoscale TiO2 have documented lung injury, inflammation, oxidative stress, and genotoxicity. Human health data are extremely scarce.
In exhaled breath condensate (EBC) and urine of 22 office employees occupationally exposed to TiO2 during their visit in the production workshops for average 14±9 min/day a panel of biomarkers of nucleic acids and proteins oxidation was studied, specifically 8-hydroxy-2-deoxyguanosine (8-OHdG), 8-hydroxyguanosine (8-OHG), 5-hydroxymethyl uracil (5-OHMeU), o-tyrosine (o-Tyr), 3-chlorotyrosine (3-ClTyr), and 3-nitrotyrosine (3-NOTyr). Examination was performed also in 14 comparable controls.
The median respirable TiO2 mass concentration in the workshops was 0.40 mg/m3, median number concentration was 2.32×104 particles/cm3 with 80% of the particles being <100 nm in diameter. All 6 markers of oxidation were elevated in EBC in factory office employees relative to controls (p<0.01). Significant association was found between their job in TiO2 production plant and 5 markers of oxidation (except 3-NOTyr) in the EBC in multivariate analysis. No elevation of markers was detected in the urine.
This pilot study suggests that even short nanoTiO2 exposure may lead to pulmonary oxidative stress, however this effect may be short-term and reversible. The clinical significance of these findings is unclear and more studies are needed.
Permanent Link: http://hdl.handle.net/11104/0271818