Cytotoxicity of poly(p-phenylenediamine)

0472634 - ÚMCH 2017 RIV SK eng J - Journal Article
Kuceková, Z. - Rejmontová, P. - Humpolíček, P. - Kašpárková, V. - Bober, Patrycja - Sáha, P. - Stejskal, Jaroslav
Cytotoxicity of poly(p-phenylenediamine).
Chemical Papers. Roč. 71, č. 2 (2017), s. 367-372. ISSN 0366-6352
R&D Projects: GA ČR(CZ) GA13-00270S
Institutional support: RVO:61389013
Keywords : cytotoxicity * poly(p-phenylenediamine) * mouse embryonic fibroblasts
OECD category: Polymer science
Impact factor: 0.963, year: 2017

Although poly(p-phenylenediamine) is an electric non-conductor, it exhibits, analogously to conducting polymers, redox activity and could, therefore, find applications in biomedicine. In the current work, the cytotoxicity of poly(p-phenylenediamine) polymer powder produced by the chemical oxidation of p-phenylenediamine with ammonium peroxydisulfate in acidic aqueous media has been studied. Primary mouse embryonic fibroblasts were used for this purpose. Interestingly, the standard methods for the determination of polymer cytotoxicity based on international standard EN ISO 10993-5 could not be applied. The reason was the interaction of polymer extracts with MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. On the basis of the evaluation of flow cytometry and micrographs taken by fluorescence microscopy on cells treated with extracts of poly(p-phenylenediamine), it can be concluded that the powder polymer possesses severe cytotoxicity. The results suggest that practical application of the polymer within biomedicine is, at the current state of knowledge, difficult, and modification of the preparation techniques and/or subsequent purification of poly(p-phenylenediamine) is needed.
Permanent Link: http://hdl.handle.net/11104/0269900