Analysis of novel members of FAHFA lipid class derived from omega 3-PUFA by LC-MS/MS and LC-MS/MS/MS

0472359 - ÚOCHB 2017 RIV CZ eng C - Conference Paper (international conference)
Rombaldová, Martina - Kuda, Ondřej - Březinová, Marie - Slavíková, Barbora - Pošta, Martin - Beier, Petr - Janovská, Petra - Veleba, J. - Kopecký Jr., J. - Kudová, Eva - Pelikánová, T. - Kopecký, Jan
Analysis of novel members of FAHFA lipid class derived from omega 3-PUFA by LC-MS/MS and LC-MS/MS/MS.
Proceedings of the 12th International Students Conference. Prague: Charles University in Prague, Faculty of Science,, 2016 - (Nesměrák, K.), s. 103-107. ISBN 978-80-7444-044-1.
[International Students Conference "Modern Analytical Chemistry" /12./. Prague (CZ), 22.09.2016-23.09.2016]
R&D Projects: GA MŠMT(CZ) LH14040; GA ČR(CZ) GB14-36804G
Institutional support: RVO:61388963 ; RVO:67985823
Keywords : branched fatty acid * lipidomics * mass spectrometry * omega 3-PUFA
Subject RIV: CC - Organic Chemistry; FB - Endocrinology, Diabetology, Metabolism, Nutrition (FGU-C)

White adipose tissue (WAT) is a complex organ with both metabolic and endocrine functions. Dysregulation of all of these functions of WAT, together with low-grade inflammation of the tissue in obesity, contributes to the development of insulin resistance and type 2 diabetes. Omega-3 polyunsaturated fatty acids (PUFA) of marine origin play an important role in resolution of inflammation and exert beneficial metabolic effects. Using LC-MS/MS method, we elucidated the structures of novel members of fatty acid esters of hydroxy fatty acids (FAHFA), which were present in murine and human serum and WAT after omega-3 PUFA supplementation in diet. These compounds contained DHA esterified to 9- and 13-hydroxyoctadecadienoic acid (HLA) termed 9-DHAHLA and 13-DHAHLA, and were synthesized in WAT. Our results document the existence of novel lipid mediators, which are involved in the beneficial anti-inflammatory effects attributed toomega-3 PUFA, in both miceandhumans.
Permanent Link: http://hdl.handle.net/11104/0269686