Fast-scan cyclic voltammetry with thiol-modified mercury electrodes distinguishes native from denatured BSA

0472036 - BFÚ 2017 RIV NL eng J - Journal Article
Černocká, Hana - Ostatná, Veronika - Paleček, Emil
Fast-scan cyclic voltammetry with thiol-modified mercury electrodes distinguishes native from denatured BSA.
Electrochemistry Communications. Roč. 61, DEC2015 (2015), s. 114-116. ISSN 1388-2481. E-ISSN 1873-1902
R&D Projects: GA ČR(CZ) GA15-15479S
Institutional support: RVO:68081707
Keywords : catalyzed hydrogen evolution * proteins * electrocatalysis
Subject RIV: BO - Biophysics
Impact factor: 4.569, year: 2015

We studied native and denatured bovine serum albumin (BSA) at bare and dithiothreithol (DTT)-modified hanging mercury drop electrode (HMDE) by fast-scan cyclic voltammetry (fsCV) and chronopotentiometric stripping (CPS) to compare these methods for their ability to recognize changes in BSA structure. Using fsCV and bare HMDE, denatured BSA could be distinguished from its native form only between 10 and 20 V/s but at lower resolution than with CPS. At DTT-HMDE denatured BSA was recognized from native BSA in a wider range of scan rates suggesting new possibilities in development of voltammetric protein structure-sensitive sensing. (C) 2015 Elsevier B.V. All rights reserved.
Permanent Link: http://hdl.handle.net/11104/0269380