Wild-type p53 binds to MYC promoter G-quadruplex

0471953 - BFÚ 2017 RIV GB eng J - Journal Article
Petr, Marek - Helma, Robert - Polášková, Alena - Krejčí, Aneta - Dvořáková, Zuzana - Kejnovská, Iva - Navrátilová, Lucie - Adámik, Matěj - Vorlíčková, Michaela - Brázdová, Marie
Wild-type p53 binds to MYC promoter G-quadruplex.
Bioscience Reports. Roč. 36, OCT2016 (2016), č. článku e00397. ISSN 0144-8463. E-ISSN 1573-4935
R&D Projects: GA ČR GA13-36108S; GA ČR GAP205/12/0466
Institutional support: RVO:68081707
Keywords : nuclease-hypersensitive element * c-terminal domain * gene-expression
Subject RIV: BO - Biophysics
Impact factor: 2.906, year: 2016

G-quadruplexes are four-stranded nucleic acid structures that are implicated in the regulation of transcription, translation and replication. Genome regions enriched in putative G-quadruplex motifs include telomeres and gene promoters. Tumour suppressor p53 plays a critical role in regulatory pathways leading to cell cycle arrest, DNA repair and apoptosis. In addition to transcriptional regulation mediated via sequence-specific DNA binding, p53 can selectively bind various non-B DNA structures. In the present study, wild-type p53 (wtp53) binding to G-quadruplex formed by MYC promoter nuclease hypersensitive element (NHE) III1 region was investigated. Wtp53 binding to MYC G-quadruplex is comparable to interaction with specific p53 consensus sequence (p53CON). Apart from the full-length wtp53, its isolated C-terminal region (aa 320-393) as well, is capable of high-affinity MYC G-quadruplex binding, suggesting its critical role in this type of interaction. Moreover, wtp53 binds to MYC promoter region containing putative G-quadruplex motif in two wtp53-expressing cell lines. The results suggest that wtp53 binding to G-quadruplexes can take part in transcriptional regulation of its target genes.
Permanent Link: http://hdl.handle.net/11104/0269314