Imaging of transfection and intracellular release of intact, non-labeled DNA using fluorescent nanodiamonds

0471415 - FZÚ 2017 RIV GB eng J - Journal Article
Petráková, Vladimíra - Benson, Veronika - Bunček, M. - Fišerová, Anna - Ledvina, Miroslav - Štursa, Jan - Cígler, Petr - Nesládek, M.
Imaging of transfection and intracellular release of intact, non-labeled DNA using fluorescent nanodiamonds.
Nanoscale. Roč. 8, č. 23 (2016), s. 12002-12012. ISSN 2040-3364. E-ISSN 2040-3372
R&D Projects: GA MZd(CZ) NV15-33094A; GA MŠMT LM2015056
Grant - others:OP VK(XE) CZ.1.07/2.3.00/20.0306
Institutional support: RVO:68378271 ; RVO:61388971 ; RVO:61389005 ; RVO:61388963
Keywords : single-particle tracking * resonance energy-transfer * sirna delivery * gene delivery * correlation spectroscopy * endosomal escape * mass-production * drug-delivery * quantum dots * living cells
Subject RIV: BM - Solid Matter Physics ; Magnetism; CC - Organic Chemistry (UOCHB-X); EE - Microbiology, Virology (MBU-M); BG - Nuclear, Atomic and Molecular Physics, Colliders (UJF-V)
Impact factor: 7.367, year: 2016

Efficient delivery of stabilized nucleic acids (NAs) into cells and release of the NA payload are crucial points in the transfection process. Here we report on the fabrication of a nanoscopic cellular delivery carrier that is additionally combined with a label-free intracellular sensor device, based on biocompatible fluorescent nanodiamond particles. The sensing function is engineered into nanodiamonds by using nitrogen-vacancy color centers, providing stable non-blinking luminescence. The device is used for monitoring NA transfection and the payload release in cells. The unpacking of NAs from a poly(ethyleneimine)-terminated nanodiamond surface is monitored using the color shift of nitrogen-vacancy centers in the diamond, which serve as a nanoscopic electric charge sensor.
Permanent Link: http://hdl.handle.net/11104/0268809