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Toward ideal separation by size-exclusion chromatography
- 1.0471173 - ÚMCH 2018 RIV NL eng J - Journal Article
Netopilík, Miloš
Toward ideal separation by size-exclusion chromatography.
Journal of Chromatography A. Roč. 1487, 3 March (2017), s. 139-146. ISSN 0021-9673. E-ISSN 1873-3778
R&D Projects: GA ČR(CZ) GC17-04258J
Grant - others:OPPK(XE) CZ.2.16/3.1.00/24504
Institutional support: RVO:61389013
Keywords : size-exclusion chromatography * separation mechanism * band broadening function
OECD category: Polymer science
Impact factor: 3.716, year: 2017
In analyses of polystyrene standards by size-exclusion chromatography for polymers of molecular weight about thirty thousand and higher, the separation improved with decreasing flow-rate is in accord with Giddings observation of transversal diffusion as a factor decreasing the broadening of the band broadening function. The variance of the elution curves is larger than it corresponds to dispersity Mw/Mn and the shape of the elution curves depends on the experimental conditions which suggests that it reflects properties of the band broadening function. The skew and the excess kurtosis of the elution curves increase near the exclusion limit in accord with theoretical prediction for the band broadening function.
Permanent Link: http://hdl.handle.net/11104/0270670
Number of the records: 1