Growth of human adipose-derived stem cells on Ti-6Al-4V alloy with various surface modifications

0470319 - FGÚ 2017 RIV CZ eng C - Conference Paper (international conference)
Kročilová, Nikola - Bačáková, Lucie - Pařízek, Martin - Havlíková, Jana - Motarjemi, H. - Molitor, M. - Gabor, R. - Marvan, J.
Growth of human adipose-derived stem cells on Ti-6Al-4V alloy with various surface modifications.
NANOCON 2015: 7th International Conference on Nanomaterials - Research and Application, Conference Proceedings. Ostrava: TANGER, spol. s r.o., 2015, s. 466-471. ISBN 978-80-87294-63-5.
[NANOCON 2015. International Conference /7./. Brno (CZ), 14.10.2015-16.10.2015]
R&D Projects: GA TA ČR(CZ) TA04011214; GA MZd(CZ) NV15-32497A
Institutional support: RVO:67985823
Keywords : bone implants * bone tissue engineering * surface roughness * stem cells * liposuction * cell proliferation
Subject RIV: EI - Biotechnology ; Bionics

Adipose-derived stem cells (ASCs) are of a great importance for bone tissue engineering. We compared ASCs obtained by liposuction under two pressures (-200 mmHg and -700 mmHg). The number and proliferation activity of cells isolated under -700 mmHg (ASC-H) were higher than in cells isolated under -200 mmHg (ASC-L). However, the ASC-L was more active in osteogenic differentiation, as manifested by a higher intensity of fluorescence of alkaline phosphatase and osteocalcin in these cells. The adhesion and growth of ASCs were then studied on Ti-6Al-4V samples either unmodified (K, roughness parameter R-a = 280 nm) or modified by shot blasting and tarnishing A, R-a = 200 nm), vibratory finishing (B, R-a = 100 nm) and vibratory finishing, shot blasting and polishing (C, R-a = 80 nm). All modified samples were more wettable than the K samples. On day 1 after seeding, the size of cell spreading area on some modified samples was lower than on K samples, namely on B samples in ASC-L, on B and C in ASC-H and on A in control MG-63 cells. On day 3, the cell number on some modified samples became higher than on K samples, namely on C samples (ASC-L), on B (ASC-H) and on B and C s (MG-63 cells). On day 7, the numbers of ASC-H and MG-63 cells on all modified samples evened out and became significantly higher than on K samples. On ASC-L samples, the highest cell numbers were obtained on A samples. Thus, all studied modifications of Ti-6Al-4V enhanced growth of ASCs and human osteoblast-like MG-63 cells.
Permanent Link: http://hdl.handle.net/11104/0267994