Characterization of WEE1 regulation mutant of the green alga Chlamydomonas reinhardtii in the response to DNA damage

0469989 - MBÚ 2017 JP eng A - Abstract
Hlavová, Monika - Čížková, Mária - Turóczy, Zoltán - Bišová, Kateřina
Characterization of WEE1 regulation mutant of the green alga Chlamydomonas reinhardtii in the response to DNA damage.
Book of Abstract of the 17th International Conference on the Cell and Molecular Biology of Chlamydomonas. Kyoto, 2016.
[17th International Conference on Cell and Molecular Biology of Chlamydomonas. 26.06.2016-01.07.2016, Kyoto]
R&D Projects: GA MŠMT(CZ) LO1416
Institutional support: RVO:61388971
Subject RIV: EA - Cell Biology

Eukaryotic cell cycle progression can be affected by various mechanisms. One of them, DNA damage checkpoint, arrests the cell cycle after incorrect DNA replication or DNA damage. To block cell cycle, DNA damage checkpoint may exploit Wee1 kinase. Our preliminary results suggested Wee1 is involved both in the regulation of mitosis and in the response to DNA damage but the surrounding gene/protein network remains unknown. We screened 20,000 insertional mutants to isolate those with altered expression of CrWEE1 upon
DNA damage and we were able to recovered 14 of them, called wer (WEE1 regulation) mutants. One of the most interesting mutants, wer12, showed very high sensitivity to zeocin. On the first sight, we did not detect any cell cycle related phenotype. The division was, however, aberrant with irregular numbers of daughter cells of different cell size. The most striking phenotype was the extremely small daughter cells resembling mat3-4 phenotype. The wer12 cell size phenotype was stable both in untreated and in zeocin-treated conditions suggesting it is unrelated to the observed DNA damage response phenotype.


Permanent Link: http://hdl.handle.net/11104/0268269