Surface plasmon resonance biosensor for detection of pregnancy associated plasma protein A2 in clinical samples

0469628 - ÚFE 2017 RIV DE eng J - Journal Article
Bocková, Markéta - Chadtová Song, Xue - Gedeonová, Erika - Levová, K. - Kalousová, M. - Zima, T. - Homola, Jiří
Surface plasmon resonance biosensor for detection of pregnancy associated plasma protein A2 in clinical samples.
Analytical and Bioanalytical Chemistry. Roč. 408, č. 26 (2016), s. 7265-7269. ISSN 1618-2642. E-ISSN 1618-2650
R&D Projects: GA ČR(CZ) GBP205/12/G118
Grant - others:AV ČR(CZ) AP1101
Program: Akademická prémie - Praemium Academiae
Institutional support: RVO:67985882
Keywords : Nanoparticles * Blood sample * Surface plasmon resonance
Subject RIV: BO - Biophysics
Impact factor: 3.431, year: 2016

Pregnancy associated plasma protein A2 (PAPP-A2) is a metalloproteinase that plays multiple roles in fetal development and post-natal growth. Here we present a novel surface plasmon resonance (SPR) biosensor for the rapid and quantitative detection of PAPP-A2 in blood samples. This biosensor uses a single surface referencing approach and a sandwich assay with functionalized gold nanoparticles for signal enhancement. We demonstrate that this SPR biosensor enables the detection of PAPP-A2 in 30 % blood plasma at levels as low as 3.6 ng/mL. We also characterize the performance of the biosensor and evaluate its cross-reactivity to a PAPP-A analogue. Finally, we utilize this SPR biosensor for the detection of PAPP-A2 in blood serum from two groups of subjects: pregnant women and healthy non-pregnant women and men
Permanent Link: http://hdl.handle.net/11104/0267452