Photocount statistics of ultra-weak photon emission from germinating mung bean

0469626 - ÚFE 2017 RIV CH eng J - Journal Article
Rafieiolhosseini, N. - Poplová, Michaela - Sasanpour, P. - Rafii-Tabar, H. - Alhossaini, M.R. - Cifra, Michal
Photocount statistics of ultra-weak photon emission from germinating mung bean.
Journal of Photochemistry and Photobiology. B - Biology Section. Roč. 162, September (2016), s. 50-55. ISSN 1011-1344. E-ISSN 1873-2682
R&D Projects: GA ČR GA13-29294S
Institutional support: RVO:67985882
Keywords : Mung bean * PMT photomultiplier tube * Abbreviations UPE ultra-weak photon emission
Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering
Impact factor: 2.673, year: 2016

Ultra-weak photon emission (UPE) is an endogenous bioluminescence phenomenon present in all biological samples with an active oxidative metabolism, even without an external pre-illumination. To verify the potential of UPE for non-invasive monitoring of metabolism and growth in germinating plants, the aim of this study was to investigate the UPE from a model system - germinating mung bean seedlings (Vigna radiata) - and analyze the statistical properties of UPE during the growth in two different conditions of imbibition (pure water and 1% sucrose). We found that in all days and in both conditions, photocount distributions of UPE time series follow the negative binomial distribution whose parameters changed during the growth due to the increasing ratio of signal-to-detector dark count. Correspondingly for both groups, the mean values of UPE increased during the seedlings growth, while the values of Fano factor show a decreasing trend towards 1 during the 6 day period. While our results do not show any significant difference in hypocotyl length and weight gain between the two groups of mung seedlings, there is an indication of a tiny suppressing effect of sucrose on UPE intensity. We believe that UPE can be exploited for a sensitive non-invasive analysis of oxidative metabolism during the plant development and growth with potential applications in agricultural research
Permanent Link: http://hdl.handle.net/11104/0267450